Journal: International Journal of Oncology
Article Title: SLC25A1 promotes lymph node metastasis of esophageal squamous cell carcinoma by regulating lipid metabolism
doi: 10.3892/ijo.2025.5721
Figure Lengend Snippet: SLC5A1 promotes lipid synthesis and affects oxidative phosphorylation of ESCC cells. (A) Intracellular lipids were stained with fluorescent lipophilic dye BODIPY 493/503. Levels of (B) free fatty acids, (C) triglycerides, and (D) cholesterol. Following glucose starvation, (E) oxygen consumption rate and (F) activity of mitochondrial respiratory chain complexes in ESCC cells was determined. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. SLC5A1, solute carrier family 25 member 1; ESCC, esophageal squamous cell carcinoma; ns, not significant; NC, Negative control; sh, short hairpin.
Article Snippet: Mitochondrial respiratory chain activity was evaluated via the Mitochondrial Respiratory Chain Complex I-V Activity Assay kit (BC0515, BC3230, BC3240, BC0945, BC1445) (Beijing Solarbio Science & Technology Co., Ltd.), according to the manufacturer's instructions.
Techniques: Phospho-proteomics, Staining, Activity Assay, Negative Control